Preface
1. Introduction: Laboratory Safety; Sterilization, Sterile Distilled Water.
2. Culture Media: Important Ingredients in Culture Media, Growth Media, Composition of Important Media.
3. Mycological Evaluation / Isolation Study Techniques: Methods of Seed Examination, Standard Methods for Mycological Evaluation of Contaminated Samples; Deep Freezing Method, Direct Microscopic Examination: Slide Culture Method, Application of Stain, Advantages of Slide Culture; Serial Dilution Agar Plate Technique, Single Spore Technique, Pour Plate Technique; Technique to Study Litter Decomposing and Leaf Spot Pathogenic Fungi
4. Identification of Fungus Grown in Culture; Microscopic Techniques of Identification: Micrometery, Slide Culture Technique, Haemocytometer, Camera Lucida; Cryopreservation; Koch’s Postulates.
5. Morpho-Taxonomic Description of Mycotoxic Fungi: Aspergillus : Section: Aspergillus, Section: Fumigati, Section: Clavati, Section: Nidulantes, Section: Versicolors, Section: Usti, Section: Terrei, and Section: Wentii ; Penicillium, Fusarium, Alternaria, Trichoderma, Trichothecium, Phoma, Phomopsis, Stachybotrys, Syncephalastrum, Chaetomium, Curvularia, Colletotrichum, and Cladosporium.
6. Mycotoxins: Aflatoxins, Kojic Acid, Fumonisins, Patulin, Ochratoxin, Stergmatocystin, Ergot, Esterogenic mycotoxins (Fusarial toxins): Trichothecenes.
7. Methods of Mycotoxin Analysis: Screening Test, Qualitative Methods: Determination of mycotoxins by Thin Layer Chromatography; Estimation of Mycotoxins: Aflatoxin, Ochratoxin, Zearalenone; Determination of Aflatoxins by Pressure Mini Column Method; Units of Concentration.
8.References
9. Glossary of Mycological Terms
